2-AMINO-2-METHYL-1,3-PROPANEDIOL AR is used as suitable buffering factor during SDS-gradient gel electrophoresis procedure. This method is followed to isolate polypeptide content in the molecular weight that ranges between 1500 to 100,000. As suitable buffering agent, 2-AMINO-2-METHYL-1,3-PROPANEDIOL AR is required to determine activity of alkaline phosphatase. As an effective spacer, it is required for isotachophoresis of protein. Vapor density of this substance is 3.63 (in air) and its ph level ranges between 7.8 to 9.7. Boiling point of this chemical is 151 degree C/10mm Hg (lit).